Extensive non-canonical phosphorylation in human cells revealed using strong-anion exchange-mediated phosphoproteomics
نویسندگان
چکیده
1 Centre for Proteome Research, Department of Biochemistry, Institute of Integrative Biology, University of Liverpool, Crown Street, Liverpool, L69 7ZB, UK 2 Department of Comparative and Functional Genomics, Institute of Integrative Biology, University of Liverpool, Crown Street, Liverpool, L69 7ZB, UK 3 Department of Biochemistry and Molecular Biology and Institute of Bioinformatics, University of Georgia, Athens, GA, USA 4 Department of Biochemistry, Institute of Integrative Biology, University of Liverpool, Crown Street, Liverpool, L69 7ZB, UK
منابع مشابه
Protein kinase C activation downregulates human organic anion transporter 1-mediated transport through carrier internalization.
Organic anion transport in intact renal proximal tubule cells in animal model systems is downregulated by treatments that activate protein kinase C (PKC). How this downregulation is achieved is not yet known. Stimulation of PKC with sn-1,2-dioctanoylglycerol resulted in strong inhibition of p-aminohippurate transport mediated by the cloned human organic anion transporter 1 (hOAT1) expressed in ...
متن کاملPhosphoproteomics Study Based on In Vivo Inhibition Reveals Sites of Calmodulin‐Dependent Protein Kinase II Regulation in the Heart
BACKGROUND The multifunctional Ca(2+)- and calmodulin-dependent protein kinase II (CaMKII) is a crucial mediator of cardiac physiology and pathology. Increased expression and activation of CaMKII has been linked to elevated risk for arrhythmic events and is a hallmark of human heart failure. A useful approach to determining CaMKII's role therein is large-scale analysis of phosphorylation events...
متن کاملPhosphoproteomics Reveals Regulatory T Cell-Mediated DEF6 Dephosphorylation That Affects Cytokine Expression in Human Conventional T Cells
Regulatory T cells (Tregs) control key events of immune tolerance, primarily by suppression of effector T cells. We previously revealed that Tregs rapidly suppress T cell receptor (TCR)-induced calcium store depletion in conventional CD4+CD25- T cells (Tcons) independently of IP3 levels, consequently inhibiting NFAT signaling and effector cytokine expression. Here, we study Treg suppression mec...
متن کاملPolysulfone-based Anion Exchange Membranes for Potential Application in Solid Alkaline Fuel Cells
In present research work, anion exchange membranes based on quaternized polysulfone with ammonium cation moieties (QAPSF) were prepared by chloromethylation, amination and alkalization. The chloromethylated polysulfone were characterized by 1HNMR spectroscopy and functionalization degree was determined according to peak area integration. Ion transport properties (ionic conductivity, ion exchang...
متن کاملComparative phosphoproteomics of zebrafish Fyn/Yes morpholino knockdown embryos.
The coordinated movement of cells is indispensable for normal vertebrate gastrulation. Several important players and signaling pathways have been identified in convergence and extension (CE) cell movements during gastrulation, including non-canonical Wnt signaling. Fyn and Yes, members of the Src family of kinases, are key regulators of CE movements as well. Here we investigated signaling pathw...
متن کامل